ADO anticorps (AA 49-261)

N° du produit ABIN4886431

Détail du produit anti-ADO anticorps

Antigène: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Épitope: AA 49-261

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ADO est non-conjugé

Application: Western Blotting (WB)

Fonction: Rabbit IgG polyclonal antibody for 2-aminoethanethiol dioxygenase(ADO) detection. Tested with WB in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for 2-aminoethanethiol dioxygenase(ADO) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: 2-aminoethanethiol dioxygenase
Protein Name: 2-aminoethanethiol dioxygenase

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Détails sur ADO

Antigène: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Autre désignation: ADO (ADO Produits)

Synonymes: anticorps C10orf22, anticorps Gm237, anticorps RGD1308233, anticorps wu:fc32g01, anticorps wu:fc52c12, anticorps zgc:101580, anticorps 2-aminoethanethiol dioxygenase, anticorps 2-aminoethanethiol (cysteamine) dioxygenase, anticorps 2-aminoethanethiol (cysteamine) dioxygenase a, anticorps ADO, anticorps Ado, anticorps LOC100351598, anticorps adoa

Sujet: Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Synonyms: ADO | C10orf22 | Cysteamine dioxygenase | Q96SZ5

ID gène: 84890