CD58 anticorps (AA 29-215)

N° du produit ABIN4886530

Cet anticorps Lapin Polyclonal détecte spécifiquement CD58 dans FACS, WB et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour CD58 anticorps (AA 29-215) (ABIN4886530)

Antigène: CD58

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD58 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-CD58 anticorps

Épitope: AA 29-215

Fonction: Anti-LFA3/CD58 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-LFA3/CD58 Antibody (ABIN4886530). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human LFA3 recombinant protein (Position: F29-R215).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Flow Cytometry, 1-3 μg/1x10⁶cells
1. Barbosa, J. A., Mentzer, S. J., Kamarck, M. E., Hart, J., Biro, P. A., Strominger, J. L., Burakoff, S. J. Gene mapping and somatic cell hybrid analysis of the role of human lymphocyte function-associated antigen-3 (LFA-3) in CTL-target cell interactions. J. Immun. 136: 3085-3091, 1986. 2. Sewell, W. A., Palmer, R. W., Spurr, N. K., Sheer, D., Brown, M. H., Bell, Y., Crumpton, M. J. The human LFA-3 gene is located at the same chromosome band as the gene for its receptor CD2. Immunogenetics 28: 278-282, 1988. 3. Wang, J., Smolyar, A., Tan, K., Liu, J., Kim, M., Sun, Z. J., Wagner, G., Reinherz, E. L. Structure of a heterophilic adhesion complex between the human CD2 and CD58 (LFA-3) counterreceptors. Cell 97: 791-803, 1999.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur CD58

Antigène: CD58

Autre désignation: CD58

Sujet:

Synonyms: Lymphocyte function-associated antigen 3,Ag3,Surface glycoprotein LFA-3,CD58,CD58,LFA3,

Tissue Specificity: Widely expressed.

Background: CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.

Poids moléculaire: 26 kDa

ID gène: 965

UniProt: P19256