CDCP1 anticorps (AA 582-667)

N° du produit ABIN4886532

Cet anticorps anti-CDCP1 est un anticorps Lapin Polyclonal détectant CDCP1 dans WB, IHC et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour CDCP1 anticorps (AA 582-667) (ABIN4886532)

Antigène: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDCP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-CDCP1 anticorps

Épitope: AA 582-667

Fonction: Anti-CDCP1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-CDCP1 Antibody Picoband® (ABIN4886532). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human CDCP1 recombinant protein (Position: R582-T667). Human CDCP1 shares 84.5% amino acid (aa) sequence identity with mouse CDCP1.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: CDCP1 CUB domain containing protein 1". 2. Scherl-Mostageer M, Sommergruber W, Abseher R, Hauptmann R, Ambros P, Schweifer N (Jul 2001). "Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer". Oncogene 20 (32): 4402-8. 3. Spassov DS, Ahuja D, Wong CH, Moasser MM (2011). "The structural features of trask that mediate its anti-adhesive functions". PLoS ONE 6 (4): e19154.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur CDCP1

Antigène: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Autre désignation: CDCP1

Sujet:

Synonyms: CUB domain-containing protein 1,Membrane glycoprotein gp140,Subtractive immunization M plus HEp3-associated 135 kDa protein,SIMA135,Transmembrane and associated with src kinases,CD318,CDCP1,TRASK,UNQ2486/PRO5773,

Tissue Specificity: Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. .

Background: CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene. It has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases). CDCP1/Trask is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines. The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype. CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.

Poids moléculaire: 130 kDa

ID gène: 64866

UniProt: Q9H5V8