Doublecortin anticorps (N-Term)

N° du produit ABIN4886559

Cet anticorps anti-Doublecortin est un anticorps Lapin Polyclonal détectant Doublecortin dans WB. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour Doublecortin anticorps (N-Term) (ABIN4886559)

Antigène: Doublecortin (DCX)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Doublecortin est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Doublecortin anticorps

Épitope: AA 74-100, N-Term

Fonction: Anti-Doublecortin/DCX Antibody Picoband®

Séquence: QSLRFHQNME LDFGHFDERD KTSRNMR

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Doublecortin/DCX Antibody Picoband® (ABIN4886559). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human Doublecortin.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Brown JP, Couillard-Després S, Cooper-Kuhn CM, Winkler J, Aigner L, Kuhn HG (December 2003). "Transient expression of doublecortin during adult neurogenesis". J. Comp. Neurol. 467 (1): 1-10. 2. Couillard-Despres S, Winner B, Schaubeck S, Aigner R, Vroemen M, Weidner N, Bogdahn U, Winkler J, Kuhn HG, Aigner L (January 2005). "Doublecortin expression levels in adult brain reflect neurogenesis".Eur. J. Neurosci. 21 (1): 1-14.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-Doublecortin (ABIN4886559)

Zhang, Du, Yang, Wang, Yu, Huang, Hu, Liu, Fu: "Granulocyte colony-stimulating factor increases the therapeutic efficacy of bone marrow mononuclear cell transplantation in cerebral ischemia in mice." dans: BMC neuroscience, Vol. 12, pp. 61, (2011) (PubMed).

Détails sur Doublecortin

Antigène: Doublecortin (DCX)

Autre désignation: DCX

Sujet:

Synonyms: Neuronal migration protein doublecortin,Doublin,Lissencephalin-X,Lis-X,DCX,DBCN, LISX,

Tissue Specificity: Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

Background: Neuronal migration protein doublecortin, also known as doublin or lissencephalin-X, is a protein that in humans is encoded by the DCX gene. This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 40 kDa

ID gène: 1641

UniProt: O43602