DHODH anticorps (N-Term)

N° du produit ABIN4886562

Cet anticorps anti-DHODH est un anticorps Lapin Polyclonal détectant DHODH dans WB et IHC. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour DHODH anticorps (N-Term) (ABIN4886562)

Antigène: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DHODH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-DHODH anticorps

Épitope: AA 132-173, N-Term

Fonction: Anti-DHODH Antibody Picoband®

Séquence: RVFRLPEDQA VINRYGFNSH GLSVVEHRLR ARQQKQAKLT E

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-DHODH Antibody Picoband® (ABIN4886562). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human DHODH, different from the related mouse sequence by four amino acids, and from the related rat sequence by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Ng, S. B., Buckingham, K. J., Lee, C., Bigham, A. W., Tabor, H. K., Dent, K. M., Huff, C. D., Shannon, P. T., Jabs, E. W., Nickerson, D. A., Shendure, J., Bamshad, M. J. Exome sequencing identifies the cause of a mendelian disorder. Nature Genet. 42: 30-35, 2010. 2. White, R. M., Cech, J., Ratanasirintrawoot, S., Lin, C. Y., Rahl, P. B., Burke, C. J., Langdon, E., Tomlinson, M. L., Mosher, J., Kaufman, C., Chen, F., Long, H. K., Kramer, M., Datta, S., Neuberg, D., Granter, S., Young, R. A., Morrison, S., Wheeler, G. N., Zon, L. I. DHODHmodulates transcriptional elongation in the neural crest and melanoma. Nature 471: 518-522, 2011.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur DHODH

Antigène: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Autre désignation: DHODH

Sujet:

Synonyms: Dihydroorotate dehydrogenase (quinone), mitochondrial,DHOdehase,1.3.5.2,Dihydroorotate oxidase,DHODH,

Tissue Specificity: Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level). .

Background: Dihydroorotate dehydrogenase (DHODH) is an enzyme that in humans is encoded by the DHODH gene on chromosome 16. The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.

Poids moléculaire: 43 kDa

ID gène: 1723

UniProt: Q02127

Pathways: Ribonucleoside Biosynthetic Process, Protein targeting to Nucleus