EpCAM anticorps (Middle Region)

N° du produit ABIN4886570

Il existe 2+ publications pour ce produit. L’anticorps anti-EpCAM Polyclonal Lapin est utilisé pour la détection de EpCAM dans des échantillons de Humain. Il a été validé pour WB, FACS, IHC, IF et ELISA.

Aperçu rapide pour EpCAM anticorps (Middle Region) (ABIN4886570)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Détail du produit anti-EpCAM anticorps

Épitope: Middle Region

Fonction: Anti-EpCAM Antibody

Séquence: ELKHKAREKP YDSKSLRTAL QKEITTRYQL DPKFITSILY ENN

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: different from the related mouse sequence by fifteen amino acids,and from the related rat sequence by sixteen amino acids.

Attributs du produit: Anti-EpCAM Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human EPCAM, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by sixteen amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL Immunofluorescence, 5 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶cells ELISA, 0.1-0.5 μg/mL

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-EpCAM (ABIN4886570)

Huang, Li, Pan, Cheng, Ren, Jia, Ma, Xu: "A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis." dans: Oncotarget, Vol. 7, Issue 36, pp. 57705-57713, (2018) (PubMed).

Zhang, Bu, Sun, Xu, Yao, He, Lai: "Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage." dans: Stem cell research & therapy, Vol. 8, Issue 1, pp. 270, (2018) (PubMed).

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: EPCAM

Sujet:

Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Gene Full Name: epithelial cell adhesion molecule

Poids moléculaire: 35-40 kDa

ID gène: 4072

UniProt: P16422