MUT anticorps (N-Term)

N° du produit ABIN4886676

Cet anticorps anti-MUT est un anticorps Lapin Polyclonal détectant MUT dans WB, IHC, IF, ICC et FACS. Adapté pour Souris, Humain et Rat.

Aperçu rapide pour MUT anticorps (N-Term) (ABIN4886676)

Antigène: MUT (Methylmalonyl Coenzyme A Mutase (MUT))

Reactivité: Souris, Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MUT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-MUT anticorps

Épitope: AA 33-65, N-Term

Fonction: Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband®

Séquence: LHQQQPLHPE WAALAKKQLK GKNPEDLIWH TPE

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband® (ABIN4886676). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human MUT, different from the related mouse sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Adjalla, C. E., Hosack, A. R., Gilfix, B. M., Lamothe, E., Sun, S., Chan, A., Evans, S., Matiaszuk, N. V., Rosenblatt, D. S. Seven novel mutations in mut methylmalonic aciduria. Hum. Mutat. 11: 270-274, 1998. 2. Berger, I., Shaag, A., Anikster, Y., Baumgartner, E. R., Bar-Meir, M., Joseph, A., Elpeleg, O. N. Mutation analysis of the MCM gene in Israeli patients with mut(0) disease. Molec. Genet. Metab. 73: 107-110, 2001. 3. Cavicchi, C., Donati, M. A., Funghini, S., la Marca, G., Malvagia, S., Ciani, F., Poggi, G. M., Pasquini, E., Zammarchi, E., Morrone, A. Genetic and biochemical approach to early prenatal diagnosis in a family with mut methylmalonic aciduria. Clin. Genet. 69: 72-76, 2006.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur MUT

Antigène: MUT (Methylmalonyl Coenzyme A Mutase (MUT))

Autre désignation: MUT

Sujet:

Synonyms: Methylmalonyl-CoA mutase, mitochondrial,MCM,5.4.99.2 ,Methylmalonyl-CoA isomerase,MUT,

Tissue Specificity: T-cell specific.

Background: Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.

Poids moléculaire: 83 kDa

ID gène: 4594

UniProt: P22033

Pathways: Monocarboxylic Acid Catabolic Process