Phosphotyrosine anticorps

N° du produit ABIN488674

Cet anticorps Souris Monoclonal détecte spécifiquement Phosphotyrosine dans WB, IP, IF et IHC. Il a été cité dans 1 publication.

Aperçu rapide pour Phosphotyrosine anticorps (ABIN488674)

Antigène: Phosphotyrosine

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phosphotyrosine est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: G104

Détail du produit anti-Phosphotyrosine anticorps

Specificité: Antibody reacts with phosphotyrosine and detects the presence ofphosphotyrosine in proteins of both unstimulated and stimulated cell lysated.

Réactivité croisée (Details): Does not cross react with phosphoserine or phosphothreonine

Homologie: antibody is not species specific

Purification: affinity purified

Immunogène: Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3-™-dimentrylaminopropyl) carbodiimide

Isotype: IgG1

Information d'application

Indications d'application: 1 : 1000 with standard ECL (WB)

Commentaires:

1 μg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 μg of rat tissue lysate by colorimetric immunoblot analysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: protein G purified IgG1 in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of thetubes.

Stock: -20 °C

Stockage commentaire: store at -20°C for one year, make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Date de péremption: 12 months

Références pour anticorps anti-Phosphotyrosine (ABIN488674)

Garton, Tonks: "Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST." dans: The Journal of biological chemistry, Vol. 274, Issue 6, pp. 3811-8, (1999) (PubMed).

Détails sur Phosphotyrosine

Antigène: Phosphotyrosine

Classe de substances: Amino Acid

Sujet: Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.