N-Cadherin anticorps (Cytoplasmic Domain) (Biotin)
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- Antigène Voir toutes N-Cadherin (CDH2) Anticorps
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
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Épitope
- Cytoplasmic Domain
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Reactivité
- Humain, Souris
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp N-Cadherin est conjugé à/à la Biotin
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Application
- Western Blotting (WB)
- Specificité
- This antibody specifically recognizes N-Cadherin at 127 kDa.
- Attributs du produit
- Synonyms: CDH2, CDHN, NCAD, Neural cadherin, CDw325, Cadherin N, N-Cadherin
- Purification
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
- Ingrédients
- incl. positive Control
- Immunogène
- Peptide conjugated to hemocyanin. Epitope: Cytoplasmic domain
- Clone
- 7-00E-009
- Isotype
- IgG1
- Top Product
- Discover our top product CDH2 Anticorps primaire
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- Indications d'application
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Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Included Positive Control: Cell lysate from untreated SH-SY5Y cells (See Ptotocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- Positive Control: Cell lysate from untreated SH-SY5Y cellsFormat: Lyophilized cell lysate from serum starved SH-SY5Y cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- PBS / 0.09 % Sodium Azide / PEG and Sucrose.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Aliquote and freeze in liquid nitrogen. Antibody can be stored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
- Date de péremption
- 12 months
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- Antigène
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
- Autre désignation
- Cadherin-2 (CDH2 Produits)
- Synonymes
- anticorps CD325, anticorps CDHN, anticorps CDw325, anticorps NCAD, anticorps N-cadherin, anticorps cadherin-2, anticorps cdhn, anticorps ncad, anticorps cdw325, anticorps CDH2, anticorps Cadherin-2, anticorps Ncad, anticorps glo, anticorps lyr, anticorps pac, anticorps wu:fb47h04, anticorps cadherin 2, anticorps cadherin 2 S homeolog, anticorps cadherin 2, type 1, N-cadherin (neuronal), anticorps CDH2, anticorps cdh2.S, anticorps cdh2, anticorps Cdh2
- Sujet
- Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal), and M- (muscle) cadherin.Synonyms: CDH2, CDHN, CDw325, Cadherin N, N-Cadherin, NCAD, Neural cadherin
- ID gène
- 1000
- UniProt
- P19022
- Pathways
- Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
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