N-Cadherin anticorps (Cytoplasmic Domain) (Biotin)

N° du produit ABIN492339

Cet anticorps anti-N-Cadherin Monoclonal Souris conjugué à Biotin (Clone 7-00E-009) (ABIN492339) détecte spécifiquement N-Cadherin dans WB. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour N-Cadherin anticorps (Cytoplasmic Domain) (Biotin) (ABIN492339)

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp N-Cadherin est conjugé à/à la Biotin

Application: Western Blotting (WB)

Clone: 7-00E-009

Détail du produit anti-N-Cadherin anticorps (Biotin)

Épitope: Cytoplasmic Domain

Specificité: This antibody specifically recognizes N-Cadherin at 127 kDa.

Attributs du produit: Synonyms: CDH2, CDHN, NCAD, Neural cadherin, CDw325, Cadherin N, N-Cadherin

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Ingrédients: incl. positive Control

Immunogène: Peptide conjugated to hemocyanin. Epitope: Cytoplasmic domain

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Included Positive Control: Cell lysate from untreated SH-SY5Y cells (See Ptotocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive Control: Cell lysate from untreated SH-SY5Y cellsFormat: Lyophilized cell lysate from serum starved SH-SY5Y cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS / 0.09 % Sodium Azide / PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Aliquote and freeze in liquid nitrogen. Antibody can be stored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.

Date de péremption: 12 months

Détails sur N-Cadherin

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Autre désignation: Cadherin-2

Sujet: Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal), and M- (muscle) cadherin.Synonyms: CDH2, CDHN, CDw325, Cadherin N, N-Cadherin, NCAD, Neural cadherin

ID gène: 1000

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane