NCOA1 anticorps (N-Term)

N° du produit ABIN492358

Cet anticorps Souris Monoclonal détecte spécifiquement NCOA1 dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Chien.

Aperçu rapide pour NCOA1 anticorps (N-Term) (ABIN492358)

Antigène: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NCOA1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 11F1

Détail du produit anti-NCOA1 anticorps

Épitope: N-Term

Specificité: AM00146PU-N specifically recognizes the N-terminus of Src in Western blot at 60 kDa.

Attributs du produit: Synonyms: c-Src, pp60c-src, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src

Purification: Subsequent Subsequent Thiophilic and Size Exclusion Chromatography.

Ingrédients: incl. positive Control

Immunogène: synthetic peptide conjugated to KLHEpitope: N-terminus

Isotype: IgG1

Information d'application

Indications d'application: ELISA: Use at 0.05 μg/mLImmunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated A431 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive Control Provided. Cell lysate from untreated A431 cellsDescription: Cell lysate from untreated A431 cells, epidermoid carcinoma (Human)Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Date de péremption: 12 months

Détails sur NCOA1

Antigène: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Autre désignation: SRC1

Sujet: Src is the best understood member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Src is activated by PDGF and other growth factors that activate transmembrane tyrosine kinase receptors as well as increased intracellular calcium or oxidative stress. Src becomes activated upon dephosphorylation of Tyr 527, leading to a conformational change of the src molecule towards an Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src

ID gène: 6714

UniProt: P12931

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha