HSPB8 anticorps

N° du produit ABIN492450

Détail du produit anti-HSPB8 anticorps

Antigène: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSPB8 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric (weak) forms in Western Blot. Does not cross react with alpha Crystalline. Exogenous HSP22 detected in dimeric form.

Attributs du produit: Synonyms: Alpha crystallin C chain, CMT2L, CRYAC, DHMN2, H11, Heat shock 22kDa protein 8, HMN2,HSB8, HspB8

Purification: Protein G Chromatography.

Immunogène: His-tagged Human recombinant HSP22

Clone: 3C12-H11

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Western blot (1/2000).1 μg/mL was sufficient for detection of Hsp22 in 20 μg of whole rat tissue extract by ECLimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur HSPB8

Antigène: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Autre désignation: HSP22 (HSPB8 Produits)

Synonymes: anticorps CMT2L, anticorps DHMN2, anticorps E2IG1, anticorps H11, anticorps HMN2, anticorps HMN2A, anticorps HSP22, anticorps MGC64408, anticorps hsc70, anticorps wu:fb01g06, anticorps wu:fi48b06, anticorps fc09c11, anticorps wu:fc04b04, anticorps wu:fc09c11, anticorps zgc:64202, anticorps AU018630, anticorps AW413033, anticorps Cryac, anticorps D5Ucla4, anticorps H11K, anticorps HSP20-like, anticorps Hsp22, anticorps heat shock protein family B (small) member 8, anticorps heat shock protein family B (small) member 8 L homeolog, anticorps heat shock protein 8, anticorps heat shock protein b8, anticorps HSPB8, anticorps hspb8.L, anticorps hspa8, anticorps hspb8, anticorps Hspb8

Sujet: Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macroautophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neurpathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).Synonyms: Alpha crystallin C chain, CMT2L, CRYAC, DHMN2, H11, HMN2, HSB8, Heat shock 22 kDa protein 8, HspB8

ID gène: 2030

UniProt: Q9UJY2