Syntaxin 6 anticorps

N° du produit ABIN492572

Détail du produit anti-Syntaxin 6 anticorps

Antigène: Syntaxin 6 (STX6)

Reactivité: Souris, Rat, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Syntaxin 6 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificité: This antibody reacts with 31 kDa membrane protein Syntaxin-6 on Western blotting and Immunoprecipitation. It is reported that this monoclonal antibody (3D10) binds to the amino-terminal 25 amino acid of Rat Syntaxin-6 (Ref.6). Detects a band of approximately 35 kDa (predicted molecular weight: 30.6 kDa).

Réactivité croisée (Details): Species reactivity (tested):Mouse, Hamster and Rat.It is reported that this clone 3D10 reacted with Human Syntaxin-6 in Reference 3.

Attributs du produit: Synonyms: Syntaxin-6, Golgi Marker

Purification: Protein-A Sepharose Chromatography of hybridoma supernatant.

Immunogène: Recombinant Rat Syntaxin-6. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/cmouse splenocyte

Clone: 3D10

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: 1 μg/mL for chemiluminescence detection system. Immunoprecipitation: 2 μg/300 μL of cell extract. Immunocytochemistry. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10%glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the cold Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 3 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7. 2 containing 1%skimmed milk as suggest in the APPLICATIONS for 1 hour at room temperature. (Theconcentration of antibody will depend on condition. )8) Wash the membrane with PBS-T [0. 05% Tween-20 in PBS] (5 minutes x 3 times). 9) Incubate the membrane with the 1: 10,000 HRP-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 10) Wash the membrane with PBS-T (10 minutes x 3 times). 11) Wipe excess buffer on the membrane, then incubate it with appropriatechemiluminescence reagent for 1 minute. 12) Remove extra reagent from the membrane by dabbing with paper towel, and seal it inplastic wrap. 13) Expose to an X-ray film in a dark room for 3 minutes. 14) Develop the film as usual. The condition for exposure and development may vary. Positive Controls: Rat brain, WR19LImmunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Syntaxin 6

Antigène: Syntaxin 6 (STX6)

Autre désignation: Syntaxin 6 / STX6 (STX6 Produits)

Synonymes: anticorps CG7736, anticorps CT23545, anticorps Dm Syx6, anticorps DmSyx6, anticorps Dmel\\CG7736, anticorps SYN6, anticorps SYX6/10, anticorps Syx, anticorps MGC78971, anticorps MGC132090, anticorps STX6, anticorps fb51b08, anticorps wu:fb51b08, anticorps zgc:110658, anticorps si:dkey-121a11.2, anticorps LOC734102, anticorps Syx6, anticorps Bmsyx6, anticorps Syntaxin-6, anticorps 2310039E05Rik, anticorps 2410005I16Rik, anticorps AA437559, anticorps AI415714, anticorps AI845861, anticorps Syntaxin 6, anticorps syntaxin 6 L homeolog, anticorps syntaxin 6, anticorps Syx6, anticorps stx6.L, anticorps STX6, anticorps stx6, anticorps Stx6

Sujet: Key requirements for protein transport are vesicular carriers with a full complement of machinery to enable them to find and fuse with the correct downstream compartment. This machinery includes the soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein (SNAP) receptors (SNAREs). SNAREs mediate diverse membrane fusion events such as neurotransmitter-filled vesicles fusing with the presynaptic plasma membrane. Syntaxin-6 is a q-SNARE found in endosomal transport vesicles. Syntaxin-6 has been shown by electron microscopy to localize mostly to the trans-Golgi network (TGN) and, to a lesser extent, to the Golgi stack.Synonyms: Golgi Marker, Syntaxin-6

ID gène: 58244

UniProt: Q9JKK1

Pathways: Synaptic Vesicle Exocytosis