Recombinant EpCAM anticorps (AA 20-60)

N° du produit ABIN4949336

Cet anticorps anti-EpCAM est un anticorps Lapin Monoclonal détectant EpCAM dans WB, FACS, IF et IHC (p). Adapté pour Humain.

Aperçu rapide pour Recombinant EpCAM anticorps (AA 20-60) (ABIN4949336)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EGP40-1555R

Détail du produit anti-EpCAM anticorps

Épitope: AA 20-60

Aucune reactivité croisée: Rat (Rattus), Souris

Purification: Protein A affinity chromatography

Immunogène: A portion of amino acids 20-60 was used as the immunogen for the recombinant EpCAM antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: Titering of the recombinant EpCAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,IF: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1),Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: EpCAM

Sujet: Recognizes a 40-43  kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.