Recombinant WT1 anticorps
Aperçu rapide pour Recombinant WT1 anticorps (ABIN4949398)
Antigène
Voir toutes WT1 AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Purification
- Protein A affinity chromatography
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Immunogène
- A recombinant protein was used as the immunogen for the recombinant WT1 antibody.
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Isotype
- IgG kappa
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Indications d'application
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Optimal dilution of the recombinant WT1 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
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Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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Agent conservateur
- Azide free
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store the recombinant WT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- WT1 (Wilms Tumor 1 (WT1))
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Autre désignation
- WT1 / Wilms Tumor 1
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Sujet
- Wilms tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
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Pathways
- Tube Formation
Antigène
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