G-CSF anticorps

N° du produit ABIN4949702

Cet anticorps anti-G-CSF est un anticorps Souris Monoclonal détectant G-CSF dans IF, FACS et IHC (p). Adapté pour Humain et Macaque.

Aperçu rapide pour G-CSF anticorps (ABIN4949702)

Antigène: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivité: Humain, Macaque

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp G-CSF est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CSF3-900

Détail du produit anti-G-CSF anticorps

Purification: Protein G affinity chromatography

Immunogène: Human recombinant full-length protein was used as the immunogen for the G-CSF antibody.

Isotype: IgG1

Information d'application

Indications d'application: Optimal dilution of the G-CSF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the G-CSF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur G-CSF

Antigène: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Autre désignation: G-CSF

Sujet: This mAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF's, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF's share 73 % sequence identity at the amino acid level.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization