Thymidine Phosphorylase anticorps

N° du produit ABIN4949902

L’anticorps Souris Monoclonal anti-Thymidine Phosphorylase a été validé pour WB, IP et IHC (p). Il convient pour détecter Thymidine Phosphorylase dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour Thymidine Phosphorylase anticorps (ABIN4949902)

Antigène: Thymidine Phosphorylase (TYMP)

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Thymidine Phosphorylase est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM322

Détail du produit anti-Thymidine Phosphorylase anticorps

Purification: Protein G affinity chromatography

Immunogène: Human recombinant full length protein was used as the immunogen for this Thymidine Phosphorylase antibody.

Isotype: IgG1

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thymidine Phosphorylase antibody to be titered up or down for optimal performance.\. IP: 1-2 μg/500 μg lysate,IHC (FFPE): 1-2 μg/mL for 30 min at RT,WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Thymidine Phosphorylase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Thymidine Phosphorylase

Antigène: Thymidine Phosphorylase (TYMP)

Autre désignation: Thymidine Phosphorylase / PD-ECGF

Sujet: Recognizes a 485 amino acid protein (55 kDa monomer / 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), also called Thymidine Phosphorylase (TP, Tymp) or Gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2