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Vimentin anticorps (AA 152-181)

Name: Vimentin anticorps (AA 152-181)
SKU: ABIN3029593
Price: 658.5 EUR
Availability: InStock

VIM Reactivité: Humain WB, IHC, ELISA, FACS Hôte: Lapin Polyclonal unconjugated

Western blot analysis of VIM antibody and A2058, A375 lysate. (Vimentin anticorps (AA 152-181))

IHC analysis of FFPE human breast carcinoma tissue stained with VIM antibody (Vimentin anticorps (AA 152-181))

IHC

VIM antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). (Vimentin anticorps (AA 152-181))

FACS

Western blot analysis of VIM antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2). (Vimentin anticorps (AA 152-181))

VIM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue. (Vimentin anticorps (AA 152-181))

IHC

FACS

8 images

N° du produit ABIN3029593

Fiche de données

Antigène Voir toutes Vimentin (VIM) Anticorps

Vimentin (VIM)
Épitope
32

18

16

16

16

14

11

8

8

7

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 152-181
Reactivité
333

127

126

61

59

44

43

37

30

22

20

14

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
Humain
Hôte
189

130

9

6

4

4

2
Lapin
Anticorps secondaires appropriés
Clonalité
197

146
Polyclonal
Conjugué
200

19

13

13

11

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
Cet anticorp Vimentin est non-conjugé
Application
282

131

100

98

95

65

49

40

39

28

23

20

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Réactivité croisée (Details)

Expected species reactivity: Bovine, Hamster, Mouse, Pig, Rat

Purification

Purified

Immunogène

A portion of amino acids 152-181 from the human protein was used as the immunogen for this VIM antibody.

Isotype

Ig Fraction

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Indications d'application

The stated application concentrations are suggested starting amounts. Titration of the VIM antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C

Stockage commentaire

For long term storage, aliquot the VIM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Antigène

Vimentin (VIM)

Autre désignation

VIM (VIM Produits)

Synonymes

anticorps CTRCT30, anticorps cb28, anticorps vime, anticorps vim, anticorps vim1, anticorps vim2, anticorps VIM, anticorps Vimentin, anticorps vim4, anticorps vimentin, anticorps vimentin L homeolog, anticorps vimentin S homeolog, anticorps VIM, anticorps Vim, anticorps vim, anticorps vim.L, anticorps vim.S

Sujet

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis