Recombinant 5-Hydroxymethylcytosine anticorps

N° du produit ABIN4953286

Détail du produit anti-5-Hydroxymethylcytosine anticorps

Antigène: 5-Hydroxymethylcytosine (5-hmC)

Type d'anticorp: Recombinant Antibody

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp 5-Hydroxymethylcytosine est non-conjugé

Application: ELISA, Dot Blot (DB), Hydroxymethylated DNA immunoprecipitation (hmeDIP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Attributs du produit: This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.

Purification: Protein A purified from animal origin-free supernatant

Immunogène: BSA-conjugated 5-hydroxymethylcytosine was used as the immunogen for this recombinant 5hmC antibody.

Clone: RM236

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the recombinant 5hmC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. Dot Blot: 0.2-1 μg/mL,ELISA: 0.1-1 μg/mL,Immunohistochemistry: 0.1-1 μg/mL (1),Immunocytochemistry: 0.5-2 μg/mL,hMeDIP: 0.2 μg/mL- 2 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the recombinant 5hmC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Détails sur 5-Hydroxymethylcytosine

Antigène: 5-Hydroxymethylcytosine (5-hmC)

Autre désignation: 5-Hydroxymethylcytosine (5hmC) (5-hmC Produits)

Classe de substances: Chemical

Sujet: This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.