LIN28B anticorps (AA 95-128)

N° du produit ABIN3031627

Cet anticorps Lapin Polyclonal détecte spécifiquement LIN28B dans WB, ELISA, FACS, IHC et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour LIN28B anticorps (AA 95-128) (ABIN3031627)

Antigène: LIN28B (Lin-28 Homolog B (LIN28B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIN28B est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-LIN28B anticorps

Épitope: AA 95-128

Réactivité croisée (Details): Expected species reactivity: Mouse

Purification: Antigen affinity purified

Immunogène: A portion of amino acids 95-128 from the human protein was used as the immunogen for this LIN28B antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the LIN28B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur LIN28B

Antigène: LIN28B (Lin-28 Homolog B (LIN28B))

Autre désignation: LIN28B

Sujet: LIN28B is a suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). [UniProt]

UniProt: Q6ZN17