LIN28B anticorps (AA 95-128)

N° du produit ABIN3031627

Détail du produit anti-LIN28B anticorps

Antigène: LIN28B (Lin-28 Homolog B (LIN28B))

Épitope: AA 95-128

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIN28B est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Réactivité croisée (Details): Expected species reactivity: Mouse

Purification: Antigen affinity purified

Immunogène: A portion of amino acids 95-128 from the human protein was used as the immunogen for this LIN28B antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the LIN28B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur LIN28B

Antigène: LIN28B (Lin-28 Homolog B (LIN28B))

Autre désignation: LIN28B (LIN28B Produits)

Synonymes: anticorps csdd2, anticorps LIN-28, anticorps CSDD2, anticorps 2810403D23Rik, anticorps D030047M17Rik, anticorps Lin-28.2, anticorps lin-28 homolog B, anticorps lin-28 homolog B S homeolog, anticorps lin-28 homolog B (C. elegans), anticorps LIN28B, anticorps lin28b, anticorps lin28b.S, anticorps Lin28b

Sujet: LIN28B is a suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). [UniProt]

UniProt: Q6ZN17