UCHL5 anticorps (AA 56-87)

N° du produit ABIN3029428

Cet anticorps Lapin Polyclonal détecte spécifiquement UCHL5 dans WB, IHC et ELISA. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour UCHL5 anticorps (AA 56-87) (ABIN3029428)

Antigène: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UCHL5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-UCHL5 anticorps

Épitope: AA 56-87

Réactivité croisée (Details): Expected species reactivity: Bovine, Pig

Purification: Purified

Immunogène: A portion of amino acids 56-87 from the human protein was used as the immunogen for this UCH37 antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the UCH37 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the UCH37 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur UCHL5

Antigène: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Autre désignation: UCH37

Sujet: Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.

UniProt: Q9Y5K5