SYNPO anticorps (C-Term)

N° du produit ABIN4956540

Cet anticorps anti-SYNPO est un anticorps Cobaye Polyclonal détectant SYNPO dans WB, IHC et IF. Adapté pour Souris.

Aperçu rapide pour SYNPO anticorps (C-Term) (ABIN4956540)

Antigène: SYNPO (Synaptopodin (SYNPO))

Reactivité: Souris

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp SYNPO est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-SYNPO anticorps

Épitope: C-Term

Réactivité croisée: Humain, Souris

Immunogène: synthetic peptides (mouse C-terminus)

Information d'application

Indications d'application: Dilution for IHC: 1:50 - 1:00, for WB 1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: serum

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Détails sur SYNPO

Antigène: SYNPO (Synaptopodin (SYNPO))

Autre désignation: synaptopodin

Sujet: The antibody reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding proteins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data), in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to posttranslational modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin. 1. The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. 2. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of murine brain. Differentiation-dependent expression in cultured hippocampal neurons.