BRI1 anticorps

N° du produit ABIN4966120

Détail du produit anti-BRI1 anticorps

Antigène: BRI1 (Protein Brassinosteroid Insensitive 1 (BRI1))

Reactivité: Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRI1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Réactivité croisée (Details): Not reactive in: Hordeum vulgare

Homologie: Brassica rapa

Immunogène: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BRI1 protein, Uniprot: O22476, TAIR: AT4G39400

Information d'application

Indications d'application: 1: 5000 (WB)

Commentaires:

Antibody was tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans. For IP: 15 μl GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Détails sur BRI1

Antigène: BRI1 (Protein Brassinosteroid Insensitive 1 (BRI1))

Autre désignation: Brassinosteroid insensitive 1 (BRI1) (BRI1 Produits)

Synonymes: anticorps ATBRI1, anticorps BIN1, anticorps BR INSENSITIVE 1, anticorps BRASSINOSTEROID INSENSITIVE 1, anticorps CABBAGE 2, anticorps CBB2, anticorps DWARF 2, anticorps DWF2, anticorps F23K16.30, anticorps F23K16_30, anticorps Leucine-rich receptor-like protein kinase family protein, anticorps BRI1

Sujet: BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1) is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase

Poids moléculaire: above 130 kDa (due to N-glycosylation)

UniProt: O22476