Pyruvate Orthophosphate Dikinase (PPDK) (AA 72-947) anticorps

N° du produit ABIN4966289

L’anticorps anti-Pyruvate Orthophosphate Dikinase (PPDK) Polyclonal Lapin est utilisé pour la détection de Pyruvate Orthophosphate Dikinase (PPDK) dans des échantillons de Arabidopsis thaliana, Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus et Zea mays. Il a été validé pour WB.

Aperçu rapide pour Pyruvate Orthophosphate Dikinase (PPDK) (AA 72-947) anticorps (ABIN4966289)

Antigène: Pyruvate Orthophosphate Dikinase (PPDK)

Reactivité: Arabidopsis thaliana, Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Zea mays

Hôte: Lapin

Clonalité: Polyclonal

Application: Western Blotting (WB)

Détail du produit

Épitope: AA 72-947

Fonction: Anti-PPDK | Pyruvate orthophosphate dikinase

Réactivité croisée (Details): Arabidopsis thaliana,Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Zea mays
Not reactive in: Cucumis sativus

Homologie: Hordeum vulagre,Kalanchoe fedtschenkoi

Purification: Immunogen affinity purified serum

Immunogène: Purified recomibinant enzyme consisting of residues 72-947 of Zea mays

Information d'application

Indications d'application: 1 : 25 000 (WB)

Commentaires:

PPDK levels inr C3 plants like Arabidopsis thaliana and Hordeum vulgare are very low and PPDK protein is very dilute in most tissues of C3 plants. To perform detection in C3 plants leaf proteins needs to be concentrated before western blot, Chastain et al. (2002).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water in 40 % glycerol to a final protein concentration of 100 ng/μl

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Détail du antigène

Antigène: Pyruvate Orthophosphate Dikinase (PPDK)

Sujet: PPDK (Pyruvate, phosphate dikinase 1) is involved in formation of phosphoenolpyruvate and activated by light-induced dephosphorylation. Inhibited by dark-induced phosphorylation. PPDK is a low-abundance enzyme in C3 plants while it is a key enzyme of C4 photosynthesis.

Poids moléculaire: 102 | 95 kDa

ID gène: 827226

UniProt: O23404