TDGF1 anticorps (Internal Region)

N° du produit ABIN498045

Cet anticorps anti-TDGF1 Polyclonal Chèvre (ABIN498045) détecte spécifiquement TDGF1 dans IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Boeuf (Vache) et Chien.

Aperçu rapide pour TDGF1 anticorps (Internal Region) (ABIN498045)

Antigène: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TDGF1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-TDGF1 anticorps

Épitope: Internal Region

Séquence: YGRNCEHDVR KEN

Specificité: Recognizes CRIPTO

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_003203.1. Genename: TDGF1

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/128000. Western blot: Preliminary experiments in Human Umbilical Cord lysates and in lysates ofcell lines HEK293 and NIH3T3 gave no specific signal but low background (at antibodyconcentration up to 2 μg/mL). Immunohistochemistry: 3-5 μg/mLIn paraffin embedded Human Placenta shows stainng assocated with nuclei in sometrophoblasts and weak cytoplasm staining in cytotrophoblasts.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20 °C.

Détails sur TDGF1

Antigène: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Autre désignation: TDGF1 / CRIPTO

Sujet: Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis. During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor beta (TGFbeta) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment. In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation. Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.Synonyms: CRGF, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1

ID gène: 6997

NCBI Accession: NP_003203

UniProt: P13385

Pathways: EGFR Signaling Pathway