ACAT1 anticorps (Internal Region)

N° du produit ABIN498064

Cet anticorps Chèvre Polyclonal détecte spécifiquement ACAT1 dans WB, IF et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Boeuf (Vache).

Aperçu rapide pour ACAT1 anticorps (Internal Region) (ABIN498064)

Antigène: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ACAT1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Détail du produit anti-ACAT1 anticorps

Épitope: AA 257-269, Internal Region

Séquence: C-KRVDFSKVPK LKT

Specificité: Recognizes Acetoacetyl Coenzyme A Thiolase/ACAT1 (257-269).

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from the internal region of Human ACAT1 according to NP_000010.1. Genename: ACAT1

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.01-0.03 μg/mL. Detects a band of Approx 45 kDa in Human Liver lysates (calculated MW of 45.5 kDaaccording to NP_000010.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ACAT1

Antigène: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Autre désignation: ACAT1 / MAT

Sujet: ACAT1, acetoacetyl CoA thiolase, plays a major role in ketone body metabolism. It is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.Synonyms: Acetoacetyl-CoA thiolase T2, Acetyl-CoA acetyltransferase mitochondrial

ID gène: 38

NCBI Accession: NP_000010

UniProt: P24752