Myogenin anticorps (C-Term)
Aperçu rapide pour Myogenin anticorps (C-Term) (ABIN498068)
Antigène
Voir toutes Myogenin (MYOG) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- C-Term
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Séquence
- C-TDAHNLHSLT SIVD
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Specificité
- Reciognizes Myogenin/MYF4 (C-term).
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Réactivité croisée (Details)
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Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human. -
Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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Immunogène
- Peptide with sequence from the C Terminus (near) of the protein sequence according to NP_002470.2. Genename: MYOG
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Indications d'application
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Peptide ELISA: Detection Limit: 1/16000. Western blot: 2-4 μg/mL. Detects a band of Approx 38 kDa in Mouse Skeletal Musclelysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
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Autre désignation
- Myogenin
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Sujet
- Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.Synonyms: BHLHC3, Class C basic helix-loop-helix protein 3, MYF4, MYOG, Myogenic factor 4
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Poids moléculaire
- Human: 25.0kDa (NP_002470.2) Mouse: 25.2kDa (NP_112466.1).
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ID gène
- 4656
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NCBI Accession
- NP_002470
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UniProt
- P15173
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Pathways
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
Antigène
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