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Myogenin anticorps (C-Term)

L’anticorps Chèvre Polyclonal anti-Myogenin a été validé pour WB et EIA. Il convient pour détecter Myogenin dans des échantillons de Humain, Souris, Rat, Porc, Chien et Boeuf (Vache).
N° du produit ABIN498068

Aperçu rapide pour Myogenin anticorps (C-Term) (ABIN498068)

Antigène

Voir toutes Myogenin (MYOG) Anticorps
Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivité

  • 114
  • 80
  • 57
  • 42
  • 31
  • 15
  • 13
  • 12
  • 1
Humain, Souris, Rat, Porc, Chien, Boeuf (Vache)

Hôte

  • 66
  • 61
  • 1
Chèvre

Clonalité

  • 68
  • 60
Polyclonal

Conjugué

  • 68
  • 8
  • 7
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Myogenin est non-conjugé

Application

  • 87
  • 61
  • 45
  • 32
  • 31
  • 22
  • 13
  • 12
  • 11
  • 11
  • 7
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Épitope

    • 32
    • 16
    • 13
    • 13
    • 10
    • 8
    • 8
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Séquence

    C-TDAHNLHSLT SIVD

    Specificité

    Reciognizes Myogenin/MYF4 (C-term).

    Réactivité croisée (Details)

    Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Bovine.
    Species reactivity (tested):Human.

    Purification

    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

    Immunogène

    Peptide with sequence from the C Terminus (near) of the protein sequence according to NP_002470.2. Genename: MYOG
  • Indications d'application

    Peptide ELISA: Detection Limit: 1/16000. Western blot: 2-4 μg/mL. Detects a band of Approx 38 kDa in Mouse Skeletal Musclelysates.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

    Autre désignation

    Myogenin

    Sujet

    Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.Synonyms: BHLHC3, Class C basic helix-loop-helix protein 3, MYF4, MYOG, Myogenic factor 4

    Poids moléculaire

    Human: 25.0kDa (NP_002470.2) Mouse: 25.2kDa (NP_112466.1).

    ID gène

    4656

    NCBI Accession

    NP_002470

    UniProt

    P15173

    Pathways

    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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