Vimentin anticorps
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- Antigène Voir toutes Vimentin (VIM) Anticorps
- Vimentin (VIM)
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Vimentin est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This antibody detects endogenous levels of Vimentin protein.
- Réactivité croisée (Details)
- Species reactivity (tested):Human.
- Purification
- Affinity chromatography using epitope-specific immunogen
- Pureté
- > 95 % (by SDS-PAGE)
- Top Product
- Discover our top product VIM Anticorps primaire
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- Indications d'application
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Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- Vimentin (VIM)
- Autre désignation
- Vimentin (VIM Produits)
- Synonymes
- anticorps CTRCT30, anticorps cb28, anticorps vime, anticorps vim, anticorps vim1, anticorps vim2, anticorps VIM, anticorps Vimentin, anticorps vim4, anticorps vimentin, anticorps vimentin L homeolog, anticorps vimentin S homeolog, anticorps VIM, anticorps Vim, anticorps vim, anticorps vim.L, anticorps vim.S
- Sujet
- Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.Synonyms: VIM
- Poids moléculaire
- approx. 57 kDa
- ID gène
- 7431
- NCBI Accession
- NP_003371
- UniProt
- P08670
- Pathways
- Caspase Cascade in Apoptosis
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