DGKE anticorps

N° du produit ABIN498319

Cet anticorps Lapin Polyclonal détecte spécifiquement DGKE dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour DGKE anticorps (ABIN498319)

Antigène: DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DGKE est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-DGKE anticorps

Specificité: This antibody detects endogenous levels of DGK-ε protein. (region surrounding Ser194)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Pureté: > 95 % (by SDS-PAGE)

Information d'application

Indications d'application: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur DGKE

Antigène: DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))

Autre désignation: DAG Kinase epsilon

Sujet: Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and -γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-?(130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-ε (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-ζ (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.Synonyms: DAGK5, DGK-epsilon, DGKE, Diacylglycerol kinase epsilon, Diglyceride kinase epsilon

Poids moléculaire: approx. 60 kDa

ID gène: 8526

NCBI Accession: NP_003638

UniProt: P52429