MELK anticorps

N° du produit ABIN498341

L’anticorps anti-MELK Polyclonal Lapin est utilisé pour la détection de MELK dans des échantillons de Humain. Il a été validé pour WB, IF et IHC (p).

Aperçu rapide pour MELK anticorps (ABIN498341)

Antigène: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MELK est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-MELK anticorps

Specificité: This antibody detects endogenous levels of MELK protein. (region surrounding asn449)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 with 0.05 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MELK

Antigène: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Autre désignation: MELK

Sujet: Maternal Embryonic Leucine Zipper Kinase (KIAA0175, HPK38) or MELK, a new member of the Snf1/AMPK family of kinases, encodes a protein with a kinase catalytic domain and a leucine zipper motif consisting of a periodic repetition of leucine residues at every seventh residue located within the N-terminal catalytic domain. This motif has been observed in myriad DNAbinding proteins and is presumed to be involved in protein-DNA interactions, and potentially protein-protein interactions. Research predicts that the gene product of MELK plays a role in the signal transduction events in the egg and early embryo. Mouse and human MELK proteins share 95 % sequence identity in the kinase domain and northern blot analysis in mouse indicates that MELK expression is restricted to spermatogonia in the testis and to oocytes in the ovary.Synonyms: KIAA0175, Maternal embryonic leucine zipper kinase, hMELK

Poids moléculaire: approx. 75 kDa

ID gène: 9833

NCBI Accession: NP_055606

UniProt: Q14680