EIF4G2 anticorps

N° du produit ABIN498384

Cet anticorps anti-EIF4G2 Polyclonal Lapin (ABIN498384) détecte spécifiquement EIF4G2 dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour EIF4G2 anticorps (ABIN498384)

Antigène: EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF4G2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-EIF4G2 anticorps

Specificité: This antibody detects endogenous levels of DAP-5 protein. (region surrounding asn65)

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohisochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur EIF4G2

Antigène: EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

Autre désignation: eIF4G2 / DAP5

Sujet: Death-associated protein 5 (DAP-5) (also known as p97 and NAT1) is a member of the eukaryotic translation initiation factor 4G (elF4G) family. DAP-5 is ubiquitously expressed and is highly conserved among species. In response to activated FAS or p53, caspase cleaves DAP-5 at position 790 to yield a C-terminal truncated protein which is capable of forming complexes with eIF4A and eIF3. DAP-5 has homology to the carboxy-terminal portion of eIF4G, but lacks the N-terminal region of eIF4G, which is responsible for association with the CAP binding protein eIF4E. By forming translationally inactive complexes with eIF4A and eIF3, but not with eIF4E, DAP-5 functions as a general repressor of translation. During apotosis, the caspase-activated DAP-5 can mediate CAP-independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP-5. DAP-5 is also required for cellular differentiation, as it controls specific gene expression pathways.Synonyms: AAG1, Death-associated protein 5, Eukaryotic translation initiation factor 4 gamma 2, FLJ41344, NAT1, eIF-4-gamma 2, p97

Poids moléculaire: approx. 90 kDa

ID gène: 1982

NCBI Accession: NP_001409

UniProt: P78344