MIP anticorps

N° du produit ABIN498527

L’anticorps anti-MIP Polyclonal Lapin est utilisé pour la détection de MIP dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IHC (p).

Aperçu rapide pour MIP anticorps (ABIN498527)

Antigène: MIP (Major Intrinsic Protein of Lens Fiber (MIP))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MIP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-MIP anticorps

Specificité: This antibody detects endogenous levels of AQP0 protein. (region surrounding Cys144)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-Chromatography using epitope-specific immunogen

Information d'application

Indications d'application: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MIP

Antigène: MIP (Major Intrinsic Protein of Lens Fiber (MIP))

Autre désignation: Aquaporin-0

Sujet: Aquaporins (AQPs) are a large family of integral membrane channel proteins that facilitate the transport of water through the cell membrane. Aquaporins are widely distributed and are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells where it functions not only as a water pore, but it is also involved in fiber-fiber adhesion and is crucial for fiber cell structure and organization. AQP0 contains an additional pore constriction, not seen in any other aquaporin structures, which may be responsible for pore gating. The closed AQP0 pore holds just three water molecules, which are spaced too far apart to form hydrogen bonds with each other. The C-terminal domain of AQP0 undergoes extensive post-translational modification, including many truncations, during lens aging due to the actions of m-Calpain, proteases or non-enzymatic mechanisms. These truncation sites may be involved in the development of cataracts.Synonyms: AQP0, Lens fiber major intrinsic protein, MIP, MIP26, MP26

Poids moléculaire: approx. 28 kDa

ID gène: 4284

NCBI Accession: NP_036196

UniProt: P30301