GUCY1A1 anticorps

N° du produit ABIN498662

L’anticorps anti-GUCY1A1 Polyclonal Lapin est utilisé pour la détection de GUCY1A1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IHC (p).

Aperçu rapide pour GUCY1A1 anticorps (ABIN498662)

Antigène: GUCY1A1 (Guanylate Cyclase 1 Soluble Subunit Alpha (GUCY1A1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GUCY1A1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GUCY1A1 anticorps

Specificité: This antibody detects endogenous levels of GCS-alpha-1 protein. (region surrounding Asp423)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur GUCY1A1

Antigène: GUCY1A1 (Guanylate Cyclase 1 Soluble Subunit Alpha (GUCY1A1))

Autre désignation: Guanylate Cyclase Soluble GUCY1A3

Sujet: Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble form, known as GCS or sGC, act as receptors for nitric oxide. The membrane-bound receptor form, known as GC, are peptide hormone receptors. GCS is a cGMP-synthesizing enzyme, which is the major receptor for the neurotransmitter nitric oxide (NO). It plays a crucial role in smooth muscle contractility, platelet reactivity and neurotransmission. GCS is a heme containing heterodimer, consisting of one 72 kDa alpha subunit and one 65 kDa beta subunit. The heme moeity mediates NO activation, and this heme group also binds carbon monoxide, which weakly stimulates the enzyme. Both NO and CO stimulation are enhanced by the allosteric activator 3-(5'-hydroxymethyl-2'furyl)-benzylindazole, YC-1. YC-1 can also stimulate GCS in a NOindependent manner. Both alpha and beta subunits are required for cGMP generation, and at least two isoforms exist for each subunit. Heterodimers consisting of alpha-1/beta-1 and alpha-2/beta-1 have been identified, and both display similar enzymatic activity.Synonyms: GCS-alpha-1, GCS-alpha-3, GUC1A3, GUCSA3, GUCY1A1, Guanylate cyclase soluble subunit alpha-3, Soluble guanylate cyclase large subunit, sGC alpha

Poids moléculaire: approx. 77 kDa

ID gène: 2982

NCBI Accession: NP_000847

UniProt: Q02108

Pathways: Myometrial Relaxation and Contraction