SP1 anticorps (pThr739)

N° du produit ABIN498740

L’anticorps anti-SP1 Polyclonal Lapin est utilisé pour la détection de SP1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB.

Aperçu rapide pour SP1 anticorps (pThr739) (ABIN498740)

Antigène: SP1 (Sp1 Transcription Factor (SP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SP1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-SP1 anticorps

Épitope: pThr739

Specificité: This antibody detects endogenous levels of Sp1 protein only when phosphorylated at Thr739.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur SP1

Antigène: SP1 (Sp1 Transcription Factor (SP1))

Sujet: Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1.Synonyms: Transcription factor Sp1

Poids moléculaire: approx. 90 kDa

ID gène: 6667

NCBI Accession: NP_003100

UniProt: P08047

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction