POLR3D anticorps

N° du produit ABIN499065

Cet anticorps anti-POLR3D Polyclonal Lapin (ABIN499065) détecte spécifiquement POLR3D dans WB. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour POLR3D anticorps (ABIN499065)

Antigène: POLR3D (Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR3D est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-POLR3D anticorps

Specificité: This antibody detects endogenous levels of POLR3D protein. (region surrounding asn314)

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography using epitope-specific immunogen

Pureté: > 95 % (by SDS-PAGE)

Information d'application

Indications d'application: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur POLR3D

Antigène: POLR3D (Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D))

Autre désignation: POLR3D

Sujet: Three genes (POLR2 J1, POLR2 J2, and POLR2 J3) form a cluster of total length of 214 530 bp in the genetic locus 7q22.1 on the long arm of chromosome 7 (contig NT_007933). The fourth gene (POLR2 J4, 31 040 bp) was localized in the cytogenetic locus 7p13 of the short arm of chromosome 7 (contig NT_007819). An analysis enabled us to refine dissimilar experimental data on the mapping of the hRPB11 subunit gene on chromosome 7. In particular, the presence of three sites of its localization according to data on hybridization with fluorescent-labeled probes (the FISH method) was explained. It was established that, upon the expression of the four human POLR2 J genes, at least 14 types of mature mRNAs encoding somewhat differing hRPB11 isoforms can be synthesized. Eleven of these mRNAs were revealed (as full-length copies or clearly identifiable fragments) in the available databases of expressed sequence tags and cDNAs. The most probable scheme of origination of the multiple genes of the POLR2 J family as a result of three consecutive segmented duplications increasing in size was proposed and substantiated.Synonyms: BN51, BN51T, DNA-directed RNA polymerase III subunit D, DNA-directed RNA polymerase III subunit RPC4, RNA polymerase III 47 kDa subunit, RNA polymerase III subunit C4, RPC4, RPC53 homolog

Poids moléculaire: approx. 38 kDa

ID gène: 661

NCBI Accession: NP_001713

UniProt: P05423