EIF3D anticorps
Aperçu rapide pour EIF3D anticorps (ABIN499071)
Antigène
Voir toutes EIF3D AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This antibody detects endogenous levels of eIF3ζ protein. (region surrounding Arg135)
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Réactivité croisée (Details)
- Species reactivity (tested):Human, Mouse.
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Purification
- Affinity Chromatography using epitope-specific immunogen.
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Indications d'application
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Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))
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Autre désignation
- EIF3D / EIF3S7
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Sujet
- Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFE, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.Synonyms: Eukaryotic translation initiation factor 3 subunit 7, Eukaryotic translation initiation factor 3 subunit D, eIF-3-zeta, eIF3 p66
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Poids moléculaire
- approx. 64 kDa
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ID gène
- 8664
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NCBI Accession
- NP_003744
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UniProt
- O15371
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Pathways
- Ribonucleoprotein Complex Subunit Organization
Antigène
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