MYO5A anticorps (Internal Region)

N° du produit ABIN499147

Cet anticorps Chèvre Polyclonal détecte spécifiquement MYO5A dans WB, IF et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Boeuf (Vache).

Aperçu rapide pour MYO5A anticorps (Internal Region) (ABIN499147)

Antigène: MYO5A (Myosin VA (MYO5A))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MYO5A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Détail du produit anti-MYO5A anticorps

Épitope: Internal Region

Séquence: C-ETKQLELDLN

Specificité: This antibody is expected to recognize both reported isoforms (NP_000250.3 and NP_001135967.1).

Réactivité croisée (Details): Species reactivity (expected):Rat, Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide sequence from the internal region of the protein sequence according to NP_000250.3, NP_001135967.1. Genename: MYO5A

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 1-3 μg/mL. This antibody detects a band of approx. 200 kDa in lysates of cell lineJurkat.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MYO5A

Antigène: MYO5A (Myosin VA (MYO5A))

Autre désignation: Myosin-Va

Sujet: Myosin-Va has been implicated in the regulation of vesicle trafficking in neurons and melanocytes. It has been suggested that class V myosins associate with membrane vesicles through their C-terminal tails. Myosin V associates with synaptic vesicles and forms a stable complex with the synaptic vesicle membrane proteins, synaptobrevin II, and synaptophysin. The dilute Myo5a gene mutation in myosin Va leads to mental retardation, variable cellular immunodeficiency and impaired melanocyte function (pigmentary dilution), known as Griscelli syndrome in humans. Myosin Va regulates melanosome distribution along microfilaments. It is found in association with the centrosome at all stages of the cell cycle. In the interphase stage, myosin Va is found in pericentriolar material. During cell division, it is found in the cytoplasm and concentrates in a trail between migrating centrioles and in the mitotic spindle poles and spindle fibers.Synonyms: Dilute myosin heavy chain, MYH12, MYO5A, Myosin heavy chain 12, Myoxin, non-muscle

Poids moléculaire: 215kDa (NP_000250.3).

ID gène: 4644

NCBI Accession: NP_000250

UniProt: Q9Y4I1

Pathways: Hormone Transport, Peptide Hormone Metabolism