PTRH2 anticorps (N-Term)

N° du produit ABIN499471

Cet anticorps Lapin Polyclonal détecte spécifiquement PTRH2 dans WB, IF et EIA. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour PTRH2 anticorps (N-Term) (ABIN499471)

Antigène: PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PTRH2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Détail du produit anti-PTRH2 anticorps

Épitope: N-Term

Specificité: Bit1 antibody was raised against a 15 amino acid peptide from near the amino-terminus of human Bit1.

Purification: Affinity chromatography purified via peptide column

Immunogène: Human Bit1 / Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2 (N-Terminus) Peptide

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western Blot: Bit1 antibody can be used for the detection of Bit1 at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur PTRH2

Antigène: PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))

Autre désignation: PTRH2 / BIT1

Sujet: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2).Synonyms: CGI-147, PTH2, Peptidyl-tRNA hydrolase 2 mitochondrial

ID gène: 51651

NCBI Accession: NP_057161

UniProt: Q9Y3E5