GOLPH3 anticorps (N-Term)

N° du produit ABIN499904

L’anticorps Lapin Polyclonal anti-GOLPH3 a été validé pour WB et EIA. Il convient pour détecter GOLPH3 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour GOLPH3 anticorps (N-Term) (ABIN499904)

Antigène: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GOLPH3 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-GOLPH3 anticorps

Épitope: N-Term

Purification: Affinity chromatography purified via peptide column

Immunogène: GOLPH3 antibody was raised against a 14 amino acid peptide from near the amino terminus of human GOLPH3.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western Blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur GOLPH3

Antigène: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Autre désignation: Golgi Phosphoprotein 3 / GOLPH3

Sujet: GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.Synonyms: Coat protein GPP34, GPP34, MIDAS, Mitochondrial DNA absence factor

ID gène: 64083

UniProt: Q9H4A6