SKA3 anticorps (C-Term)

N° du produit ABIN500732

Détail du produit anti-SKA3 anticorps

Antigène: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Épitope: C-Term

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SKA3 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody detects SKA3.

Réactivité croisée (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogène: SKA3 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human SKA3.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur SKA3

Antigène: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Autre désignation: SKA3 (SKA3 Produits)

Synonymes: anticorps C13orf3, anticorps RAMA1, anticorps RGD1307201, anticorps C12H13ORF3, anticorps fc23d11, anticorps si:rp71-68n21.13, anticorps wu:fc23d11, anticorps rama1, anticorps F630043A04Rik, anticorps spindle and kinetochore associated complex subunit 3, anticorps spindle and kinetochore associated complex subunit 3 L homeolog, anticorps SKA3, anticorps Ska3, anticorps ska3, anticorps ska3.L

Sujet: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.Synonyms: C13orf3, RAMA1, Spindle and kinetochore-associated protein 3

ID gène: 221150

NCBI Accession: NP_659498

UniProt: Q8IX90