MBD1 anticorps (AA 291-586)

N° du produit ABIN5027346

Détail du produit anti-MBD1 anticorps

Antigène: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Épitope: AA 291-586

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MBD1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purification: Protein G Chromatography

Immunogène: A partial length recombinant MBD1 protein (amino acids 291-586) was used as the immunogen for this antibody.

Clone: ABM15H2

Isotype: IgG1 kappa

Information d'application

Indications d'application: WB: 2-4 μg/mL, IHC: 5 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Détails sur MBD1

Antigène: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Autre désignation: MBD1 (MBD1 Produits)

Synonymes: anticorps CXXC3, anticorps PCM1, anticorps RFT, anticorps Cxxc3, anticorps CCDC11, anticorps methyl-CpG binding domain protein 1, anticorps MBD1, anticorps Mbd1

Sujet: MBD1 (Methyl-CpG-Binding Domain Protein 1) protein is a primary candidate for the readout of DNA methylation as it recruits chromatin remodelers, histone deacetylases and methylases to methylated DNA associated with gene repression. This protein, a member of a transcriptional repressor family MBD, is predominantly expressed in neurons. MBD protein binding requires both functional MBD domains and methyl-CpGs, however, some MBD proteins also bind unmethylated DNA and active regulatory regions via alternative regulatory domains or interaction with the NuRD/Mi-2 (Nucleosome Remodeling Deacetylase) complex members. The CXXC3 domain of MBD1 makes it a unique member of the MBD family due to its affinity to unmethylated DNA. MBD1 acts as an epigenetic regulator via different mechanisms, such as the formation of the MCAF1/MBD1/SETDB1 complex or the MBD1-HDAC3 complex. It also plays an important role in disease progression, contributes to the drug resistance of PC cells, however, the mechanism underlying the drug resistance endowed by MBD1 remains unknown.

Poids moléculaire: 66 kDa

ID gène: 4152

UniProt: Q9UIS9