Hexanoyl-Lysine Adduct (HEL) anticorps (Biotin)
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- Antigène
- Hexanoyl-Lysine Adduct (HEL)
- Reactivité
- Toutes les espèces
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Biotin
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Application
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificité
- Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
- Purification
- Protein G Purified
- Immunogène
- Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
- Clone
- 5D9
- Isotype
- IgG1
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- Indications d'application
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- FACS (1:50)
- FCM (1:50)
- optimal dilutions for assays should be determined by the user.
- Commentaires
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A 1:1000 dilution of ABIN5067246 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 μg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
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- Antigène
- Hexanoyl-Lysine Adduct (HEL)
- Autre désignation
- Hexanoyl-Lysine adduct
- Sujet
- Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.
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