Methylglyoxal (MG) anticorps (Biotin)

N° du produit ABIN5067390

Détail du produit

Antigène: Methylglyoxal (MG)

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Specificité: Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, MDA, 4-HHE, 4-HNE, or Crotonaldehyde modified proteins.

Purification: Protein G Purified

Immunogène: Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).

Clone: 9E7

Isotype: IgG2a

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:50)
• FACS (1:50)
• FCM (1:50)
• ELISA (1:1000)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN5067390 was sufficient for detection of Methylglyoxal in 0.5 μg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détail du antigène

Antigène: Methylglyoxal (MG)

Autre désignation: Methylglyoxal

Classe de substances: Chemical

Sujet: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Methylglyoxal is an alpha-oxoaldehyde generated from lipid peroxidation that reacts with proteins to form adducts(1). It reacts with free amino acids and protein residues to generate advanced glycation endproducts (AGEs) and crosslinks DNA polymerase and substrate DNA to severely inhibit DNA replication (2).