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Dityrosine anticorps (PerCP)

DT WB, ELISA, ICC, IF, FACS Hôte: Souris Monoclonal 10A6 PerCP
N° du produit ABIN5067484
  • Antigène Tous les produits Dityrosine (DT)
    Dityrosine (DT)
    Hôte
    • 11
    Souris
    Clonalité
    • 11
    Monoclonal
    Conjugué
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Dityrosine est conjugé à/à la PerCP
    Application
    • 11
    • 10
    • 9
    • 9
    • 9
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Specificité
    Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
    Purification
    Protein G Purified
    Immunogène
    Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
    Clone
    10A6
    Isotype
    IgG1
  • Indications d'application
    • WB (1:1000)
    • ICC/IF (1:50)
    • FACS (1:50)
    • FCM (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:1000 dilution of ABIN5067484 was sufficient for detection of dityrosine in 1 μg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    Dityrosine (DT)
    Autre désignation
    Dityrosine (DT Produits)
    Classe de substances
    Dipeptide
    Sujet
    ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).
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