Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

STON1 anticorps (AA 529-620)

STON1 Reactivité: Humain ELISA, IF Hôte: Souris Monoclonal 1F3 unconjugated
N° du produit ABIN524426
  • Antigène Voir toutes STON1 Anticorps
    STON1 (Stonin 1 (STON1))
    Épitope
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 529-620
    Reactivité
    • 21
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 18
    • 3
    Souris
    Clonalité
    • 19
    • 2
    Monoclonal
    Conjugué
    • 14
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp STON1 est non-conjugé
    Application
    • 13
    • 8
    • 3
    • 2
    • 1
    • 1
    ELISA, Immunofluorescence (IF)
    Fonction
    Mouse monoclonal antibody raised against a partial recombinant SBLF.
    Séquence
    SLKSVVVVQG AYVELQAFVN MASLAQRSSY AGSLRSCDNI RIHFPVPSQW IKALWTMNLQ RQKSLKAKMN RRACLGSLQE LESEPVIQVT VG
     Réactivité croisée
    Humain
    Attributs du produit
    Antibody Reactive Against Recombinant Protein.
    Immunogène
    SBLF (NP_006864, 529 a.a. ~ 620 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
    Clone
    1F3
    Isotype
    IgG2a
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Validation #101553 (Immunofluorescence)
    'Independent Validation' signe
    by
    Lab of Prof. Dr. S.A. Kushner, Department of Psychiatry, Erasmus University Medical Center
    No.
    #101553
    Date
    12.01.2018
    Antigène
    STON1
    Numéro du lot
    08242-1F3
    Application validée
    Immunofluorescence
    Contrôle positif
    U-373 cell line (human glioblastoma astrocytoma)
    Contrôle négative
    secondary only
    Conclusion

    The STON1 antibody ABIN524426 specifically labels the targeted antigen in sample in IF.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN524426
    Anticorps secondaire
    donkey anti-mouse A647-conjugated antibody (Sanbio, 715-605-150)
    Full Protocol
    • Grow U-373 cells (human glioblastoma astrocytoma) in DMEM, high glucose, glutamax (Gibco, product number: 31966-021, lot number: 1894785 supplemented with Fetal Bovine Serum (Gibco, 10082-147, lot 1907451) and penicillin streptomycin (Gibco, 15140-122, lot 1910857), at 37°C and 5% CO2 in 2ml on a coverslips.
    • Transfect cells with aCSPG4-GFP expression plasmid using X-tremeGENE HP (Sigma) accoding to the manufacturer’s recommendations.
    • Fix cells on coverslips in 4% PFA for 20min at RT.
    • Wash cells 3x for 5min with PBS.
    • Wash coverslips for 5min with staining solution (50mM TrisHCl pH7.4, 154mM NaCl, 0.25% w/v gelatine, 5% v/v triton x-100).
    • Incubate cells with primary mouse anti-human STON1 antibodylavivirus (antibodies-online, ABIN524426, lot 08242-1F3) diluted 1:100 in staining solution ON at 4°C.
    • Wash cells 3x for 5min with PBS.
    • Incubate cells with secondary donkey anti-mouse A647-conjugated antibody (Sanbio, 715-605-150) diluted 1:200 in staining solution for 1.5h at RT.
    • Wash cells 2x for 5min with PBS.
    • DAPI counterstain.
    • Mount coverslips on glass slides in Mowiol 4-88 antifade reagent (Sigma, 81381, lot BCBL1789V).
    • Image acquisition with LSM 700, 63x magnification, 1024x1024 resolution.
    Notes
    • ABIN524426 was used in cells overexpressing CSPG4 fused to GFP. Stonin is expected to be abundantly expressed in the cells.

    • No signal was detected in the secondary antibody only control (not shown).

  • Buffer
    In 1x PBS, pH 7.4
    Conseil sur la manipulation
    Aliquot to avoid repeated freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Antigène
    STON1 (Stonin 1 (STON1))
    Autre désignation
    STON1 (STON1 Produits)
    Synonymes
    anticorps SALF, anticorps SBLF, anticorps STN1, anticorps STNB1, anticorps DKFZP469K176, anticorps 4921524J06Rik, anticorps C86231, anticorps Salf, anticorps Sblf, anticorps stonin 1, anticorps STON1, anticorps ston1, anticorps Ston1
    Sujet
    Full Gene Name: stonin 1
    Synonyms: DKFZp781K2462,MGC149803,MGC149804,SBLF,STN1,STNB1,stoned-b1
    ID gène
    11037
    NCBI Accession
    NM_006873
Vous êtes ici:
Support technique