5'-Nucleotidase, Cytosolic II (NT5C2) (AA 1-561) anticorps

Détails pour le produit réf. ABIN524900
Antigène
  • nt5c2
  • wu:fc36f11
  • wu:fc45b10
  • GMP
  • NT5B
  • PNT5
  • cN-II
  • NT5C2
  • 2010002I23Rik
  • CnII
  • ENSMUSG00000025039
  • Gm9751
  • Gmp
  • Nt5b
  • Pnt5
  • 5'-nucleotidase, cytosolic II
  • 5'-nucleotidase, cytosolic IIa
  • NT5C2
  • nt5c2.L
  • nt5c2
  • nt5c2a
  • Nt5c2
  • surE2
Épitope
AA 1-561
8
8
8
4
2
2
2
2
1
1
1
1
1
Reactivité
Humain
60
29
24
1
Hôte
Souris
57
3
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
6
6
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
43
31
13
9
9
5
3
3
1
1
1
Options
Immunogène NT5C2 (AAH01595, 1 a.a. ~ 561 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E
Clone 3C1
Isotype IgG2a kappa
 Réactivité croisée Humain
Antigène
Autre désignation NT5C2 (NT5C2 Antibody Extrait)
Sujet Full Gene Name: 5'-nucleotidase, cytosolic II
Synonyms: GMP,NT5B,PNT5,cN-II
ID gène 22978
Indications d'application Optimal working dilution should be determined by the investigator.
Commentaires

Product Quality tested by: Antibody Reactive Against Recombinant Protein.

Restrictions For Research Use only
Buffer In 1x PBS, pH 7.4
Conseil sur la manipulation Aliquot to avoid repeated freezing and thawing.
Stock -20 °C
Stockage commentaire Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.
Images (Fournisseur)
Immunostaining (ISt) image for anti-5'-Nucleotidase, Cytosolic II (NT5C2) (AA 1-561) antibody (ABIN524900) Immunoperoxidase of monoclonal antibody to NT5C2 on formalin-fixed paraffin-embedded ...
Western Blotting (WB) image for anti-5'-Nucleotidase, Cytosolic II (NT5C2) (AA 1-561) antibody (ABIN524900) Western Blot analysis of NT5C2 expression in transfected 293T cell line by NT5C2 mono...
Immunofluorescence (IF) image for anti-5'-Nucleotidase, Cytosolic II (NT5C2) (AA 1-561) antibody (ABIN524900) Immunofluorescence of monoclonal antibody to NT5C2 on HeLa cell. [antibody concentrat...
 image for anti-5'-Nucleotidase, Cytosolic II (NT5C2) (AA 1-561) antibody (ABIN524900) Western Blot detection against Immunogen (87.45 KDa) .
Produit citée dans: Cividini, Tozzi, Galli, Pesi, Camici, Dumontet, Jordheim, Allegrini: "Cytosolic 5'-nucleotidase II interacts with the leucin rich repeat of NLR family member Ipaf." dans: PLoS ONE, Vol. 10, Issue 3, pp. e0121525, 2015 (PubMed).

Jordheim, Puy, Cros-Perrial, Peyrottes, Lefebvre, Périgaud, Dumontet: "Determination of the enzymatic activity of cytosolic 5'-nucleotidase cN-II in cancer cells: development of a simple analytical method and related cell line models." dans: Analytical and bioanalytical chemistry, Vol. 407, Issue 19, pp. 5747-58, 2015 (PubMed).

Cividini, Cros-Perrial, Pesi, Machon, Allegrini, Camici, Dumontet, Jordheim, Tozzi: "Cell proliferation and drug sensitivity of human glioblastoma cells are altered by the stable modulation of cytosolic 5'-nucleotidase II." dans: The international journal of biochemistry & cell biology, Vol. 65, pp. 222-9, 2015 (PubMed).

Kulkarni, Karlsson, Szekeres, Chibalin, Krook, Zierath: "Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle." dans: The Journal of biological chemistry, Vol. 286, Issue 40, pp. 34567-74, 2011 (PubMed).

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