SMT1 anticorps

N° du produit ABIN5326722

Cet anticorps Lapin Polyclonal détecte spécifiquement SMT1 dans IL et WB. Il présente une réactivité avec des échantillons de Arabidopsis thaliana. Il a été cité dans 2+ publications.

Aperçu rapide pour SMT1 anticorps (ABIN5326722)

Antigène: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Reactivité: Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Détail du produit anti-SMT1 anticorps

Fonction: Anti-SMT1 | Sterol methyltransferase 1

Réactivité croisée (Details): Arabidopsis thaliana
Not reactive in: Hordeum vulgare, Triticum aestivum, Solanum lycopersicum, Withania somnifera

Homologie: Amborella trichopoda,Brassica napus,Brassica rapa,Capsella rubella,Citrus clementina,Eutrema salsugineum,Glycine max,Glycine soja,Gossypium mexicanum,Medicago truncatula,Populus trichopocarpa,Prunus persica,Ricinus communis,Theobroma cacao,Vitis vinifera

Purification: Immunogen affinity purified serum

Immunogène: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 UniProt: Q9LM02

Information d'application

Indications d'application: 1 : 50-1 : 100 (IL), 1 : 500-1 : 1000 (WB)

Commentaires:

SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Références pour anticorps anti-SMT1 (ABIN5326722)

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." dans: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

Boutté, Frescatada-Rosa, Men, Chow, Ebine, Gustavsson, Johansson, Ueda, Moore, Jürgens, Grebe: "Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis." dans: The EMBO journal, Vol. 29, Issue 3, pp. 546-58, (2010) (PubMed).

Détails sur SMT1

Antigène: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Autre désignation: Sterol methyltransferase 1 (SMT1)

Sujet:

Background: SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. The protein is highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD

Additional information: SMT1 is an integral membrane protein of ER (Boutte et al., 2009) while BiP is a membrane associated protein.Endogenous SMT1 is expressed at very low levels in root epidermis and root cap as compare to cortex and endodermis and therefore this can contribute to SMT1 detection problems.

Poids moléculaire: 38 kDa

ID gène: 831216

UniProt: Q9LM02