SMT1 anticorps

N° du produit ABIN5326722

Cet anticorps Lapin Polyclonal détecte spécifiquement SMT1 dans IL et WB. Il présente une réactivité envers Arabidopsis thaliana et a été mentionné dans 2+ publications.

Aperçu rapide pour SMT1 anticorps (ABIN5326722)

Antigène: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Reactivité: Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Détail du produit anti-SMT1 anticorps

Réactivité croisée (Details): Not reactive in: Hordeum vulgare, Triticum aestivum, Withania somnifera

Homologie: Amborella trichopoda, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera,

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 38 kDa

Purification: affinity purified

Immunogène: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 Q9LM02, At5g13710

Information d'application

Indications d'application: 1: 500 - 1:1 000 with ECL Advance or + (WB), 1: 50 - 1: 100 (IL)

Commentaires:

SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Références pour anticorps anti-SMT1 (ABIN5326722)

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." dans: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

Boutté, Frescatada-Rosa, Men, Chow, Ebine, Gustavsson, Johansson, Ueda, Moore, Jürgens, Grebe: "Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis." dans: The EMBO journal, Vol. 29, Issue 3, pp. 546-58, (2010) (PubMed).

Détails sur SMT1

Antigène: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Autre désignation: Sterol methyltransferase 1 (SMT1)

Sujet: AGI Code: At5g13710
SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD

Poids moléculaire: 38 kDa

UniProt: Q9LM02