Nanog anticorps (Middle Region)

N° du produit ABIN5518779

Cet anticorps Lapin Polyclonal détecte spécifiquement Nanog dans WB et FACS. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Nanog anticorps (Middle Region) (ABIN5518779)

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nanog est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-Nanog anticorps

Épitope: AA 115-155, Middle Region

Fonction: Anti-Nanog Antibody

Séquence: QRQKYLSLQQ MQELSNILNL SYKQVKTWFQ NQRMKSKRWQ K

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Nanog Antibody (ABIN5518779). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human Nanog, different from the related mouse sequence by three amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Nanog

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Autre désignation: NANOG

Sujet:

Synonyms: Homeobox protein NANOG,Homeobox transcription factor Nanog,hNanog,NANOG,

Tissue Specificity: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. .

Background: NANOG (pron. nanOg) is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. In humans, this protein is encoded by the NANOG gene. It is mapped to 12p13.31. NANOG is thought to be a key factor in maintaining pluripotency. Moreover, NANOG is also thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. The < a href="https://www.bosterbio.com/human-nanog-recombinant-protein-protq9h9s0-boster.html" style="color:#EA8D28">NANOG protein has been found to be a transcriptional activator for the Rex1 promoter, playing a key role in sustaining Rex1 expression. Knockdown of NANOG in embryonic stem cells results in a reduction of Rex1 expression, while forced expression of NANOG stimulates Rex1 expression.

Poids moléculaire: 48 kDa

ID gène: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance