HPSE anticorps (AA 206-457)

N° du produit ABIN5518844

Cet anticorps anti-HPSE est un anticorps Lapin Polyclonal détectant HPSE dans WB. Adapté pour Souris. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour HPSE anticorps (AA 206-457) (ABIN5518844)

Antigène: HPSE (Heparanase (HPSE))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HPSE est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-HPSE anticorps

Épitope: AA 206-457

Fonction: Anti-Heparanase 1/Hpse Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Heparanase 1/Hpse Antibody Picoband® (ABIN5518844). Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived mouse Heparanase 1 recombinant protein (Position: K206-K457). Mouse Heparanase 1 shares 80.2% and 96.4% amino acid (aa) sequence identity with human and rat Heparanase 1, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Mouse
1. Hulett MD, Freeman C, Hamdorf BJ, Baker RT, Harris MJ, Parish CR (July 1999), "Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis", Nature medicine 5 (7): 803-9. 2. Nakajima M, Irimura T, Nicolson GL. (1988), "Heparanases and tumor metastasis", J. Cell. Biochem. 36 (2): 157-167. 3. Toyoshima, M., Nakajima, M. : Human heparanase: purification, characterization, cloning, and expression. J. Biol. Chem. 274: 24153-24160, 1999. 4. Vlodavsky I, Friedmann Y, Elkin M, Aingorn H, Atzmon R, Ishai-Michaeli R, Bitan M, Pappo O, Peretz T, Michal I, Spector L, Pecker I (July 1999), "Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis", Nature medicine 5 (7): 793-802. 5. Vlodavsky I, Goldshmidt O, Zcharia E, et al. (2003), "Mammalian heparanase: involvement in cancer metastasis, angiogenesis and normal development.", Semin. Cancer Biol. 12 (2): 121-9.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-HPSE (ABIN5518844)

Qin, Xu, Qi, Guo, Wang, Xu, Qiao, Gu, Niu: "Evaluation of the C-domain of heparanase during AGE-induced macrophage inflammatory response." dans: Experimental and therapeutic medicine, Vol. 14, Issue 2, pp. 1017-1022, (2017) (PubMed).

Huining, Yi, Dihong, Yifeng, Man, Ting, Jingting: "Inhibition of choriocarcinoma by Fe3O4-dextran-anti-β-human chorionic gonadotropin nanoparticles containing antisense oligodeoxynucleotide of heparanase." dans: International journal of nanomedicine, Vol. 8, pp. 4371-8, (2014) (PubMed).

Détails sur HPSE

Antigène: HPSE (Heparanase (HPSE))

Autre désignation: Hpse

Sujet:

Synonyms: Heparanase,3.2.1.166,Endo-glucoronidase,Heparanase 8 kDa subunit,Heparanase 50 kDa subunit,Hpse,Hpa,

Tissue Specificity: Expressed in skin, mainly in the stratum granulosum and the first layer of the stratum corneum in the upper part of the epidermis. Also detected in hair follicles and in sebaceous glands. .

Background: Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate and has been implicated in inflammation and tumor angiogenesis and metastasis. The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.

Poids moléculaire: 65 kDa

ID gène: 15442

Pathways: Glycosaminoglycan Metabolic Process