Vinculin anticorps (AA 930-1134)

N° du produit ABIN5518889

Cet anticorps anti-Vinculin est un anticorps Lapin Polyclonal détectant Vinculin dans WB. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour Vinculin anticorps (AA 930-1134) (ABIN5518889)

Antigène: Vinculin (VCL)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Vinculin est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Vinculin anticorps

Épitope: AA 930-1134

Fonction: Anti-Vinculin/VCL Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Vinculin/VCL Antibody Picoband® (ABIN5518889). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Vinculin recombinant protein (Position: E930-Q1134). Human Vinculin shares 87.7% amino acid (aa) sequence identity with both mouse and rat Vinculin.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Ezzell RM, Goldmann WH, Wang N, Parashurama N, Parasharama N, Ingber DE (1997). "Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton". Exp. Cell Res. 231 (1): 14-26. 2. Goldmann WH, Ingber DE (2001). "Intact vinculin protein is Require for control of cell shape, cell mechanics, and rac-Dependent Lamellipodia Formation". Biochemical and Biophysical Research Communications. 290 (2): 749-755. 3. Xu W, Baribault H, Adamson ED (1998). "vinculin knockout results in heart and brain defects during embryonic development". Development. 125(2): 327-337.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-Vinculin (ABIN5518889)

Cong, Fu, Zhang, Zhao, Zhang: "Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells." dans: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 210-216, (2018) (PubMed).

Shen, Lu, Li, Zhang, Zhu: "Synthesis and characterization of biodegradable polyurethane for hypopharyngeal tissue engineering." dans: BioMed research international, Vol. 2015, pp. 871202, (2015) (PubMed).

Su, Liu, Ma, Wang, E, Liu: "Enhancement of periodontal tissue regeneration by transplantation of osteoprotegerin-engineered periodontal ligament stem cells." dans: Stem cell research & therapy, Vol. 6, pp. 22, (2015) (PubMed).

Détails sur Vinculin

Antigène: Vinculin (VCL)

Autre désignation: VCL

Sujet:

Synonyms: Vinculin,Metavinculin,MV,VCL,

Tissue Specificity: Metavinculin is muscle-specific.

Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Poids moléculaire: 124 kDa

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2